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Brick Wall of Science By Bonnie Lin

If you are coming here to look for answers on why to enter the world of science, then I am afraid this will disappoint you.

The truth is, as a rising junior pursuing a bachelor degree in biomolecular science, I don’t have a definite answer for you either.

As if being a first-generation college student is not hard enough, I am a woman in an engineering school. Now, I am not talking about the struggle of how women are being outnumbered by men in the field of STEM because I can see this slowly changing around me. I am talking about being a woman pursuing a STEM degree in my family. My sister, who is 10 years older than me and the first one to attend college, pursued a business degree like many of my other female cousins. Growing up, I have always looked up to my sister, and often followed her examples. Entering high school, I had my future all planned out. I decided to major in accounting when I applied to college. Why? The answer is quite simple: It is easy to find a job and make decent money; it was a common major for women to pursue; and I had always been pretty good at math (at least in high school). Having planned everything out, I shocked not only my parents but myself as well when I told them I wanted to pursue biomolecular science. When they asked me why, I couldn’t come up with an answer. Their doubt and uncertainty in my decision added on to my uncertainty of whether or not I chose the right path.

Two years into college, I still can not tell you for sure if science is the best field for me to pursue. But I can tell you for sure that I do not regret my decision. Attending so many lectures and talks from great professors whose research have astonishing results,  opened new doors to what science can lead to and can help achieve. Yes, there can be failed experiments, and it may be years and years of frustration before achieving a desired result or breakthrough. Maybe it is this unpredictability that draws me towards science. How great would one feel when the many failed experiments and long hours in the lab finally lead to something?

Maybe it is this feeling of pride that I am looking forward to. This surge of pride when suddenly all my years of challenges and struggle paved the way towards discoveries that people would appreciate. At the end of many talks, I kept thinking to myself, how amazing it would be to actually be the one standing on the stage to talk about my achievements and the chance to inspire others.

Although I have such aspirations, when I look around me to see what my peers have accomplished, I feel I still have a long way to go. I don’t have a 4.0 GPA; I am not the brightest of my class; and I have never been exposed to research (until the Summer Research Program that offered me an opportunity to gain insight in research through Professor Jin Montclare’s Lab) and I have absolutely no idea where to start. It was then that I came across Randy Pausch’s “Last Lecture: Achieving Your Childhood Dreams” where one of his many lessons still lingered in my head. “The brick walls are there for a reason. The brick walls are there to give us a chance to show how badly we want something”. I have a brick wall in front of me right now. My brick wall is the sense of uncertainty and doubt I have in myself. While it may appear challenging right now, I know that one day I will be able to break through and prove to myself how badly I have wanted it all along.

If there is only one thing I want you to get out from this blog, it is that sometimes it is okay to be unsure and have questions on what you are passionate about and what you really want to do. This just adds on to the excitement and appreciation when you finally find out what you want to do. My ultimate request to you: Don’t get intimidated by how hard or how impossible something might be, maybe years later you would be on the stage with an audience applauding at your achievements. Instead of pursuing a career that may seem the easy way out, chase after a career you imagine you would be happy in, and most importantly, the career you do not regret even if it intimidates you at this moment.

This blog is mostly my effort to remind myself that I still have my brick wall to break and answers to seek. Is science what I really badly want? Maybe you could ask me 10 years later and see whether I still have this brick wall in front of me.

_Bonnie Lin (@BonnieL17279208)

 

Works Cited:

Kabakou, Maksim. Science Concept: Painted Red Flask icon on Black Brick wall background

with Hand Drawn Science Icons” Issue ID 112170700.

https://www.123rf.com/photo_112170700_science-concept-painted-red-flask-icon-on-black-brick

-wall-background-with-hand-drawn-science-icons.html

Pausch, Randy, and Jeffrey Zaslow. The Last Lecture.Hachette Books, 2018

Are GMOs that scary? by Jacob Kronenberg

Jacob Kronenberg kayaking with his mom, Heidi.

           Working with genetic engineering means I have to field a lot of questions when I’m home for the holidays. My health-conscious mother always makes sure to buy organic, free-range, “chemical-free” products, so when food labeled GMO-free started popping up, she made sure to get that too. In the produce section at Whole Foods I’d hear, “Jake, can you believe what those scientists do, with all this unnatural, genetically-modified Frankenstein crap they’re trying to feed us? When I was little, we just had regular strawberries and regular corn, none of these humongous GMO plants. Not to mention how Big Pharma is making mutant drugs to put in people’s bodies… C’mon, you’re a scientist now, what do you think of it?”

           This is a loaded question. All scientists are ambassadors to the community, and it’s important to dispel myths about our fields, especially when it comes to widely misunderstood topics. From zombie movies to GATTACA, genetic engineering has always been painted in a dystopic light. It also doesn’t help that agricultural use of GMOs doesn’t exactly have a clean record. Chemical-resistant crops have encouraged the use of harmful pesticides, most famously Roundup, and many large ag-tech companies have aggressive policies gatekeeping access to their designer crops. With information and misinformation obscuring knowledge of science, it can be tough to know what to say.

           I tell people who ask my thoughts on genetic engineering not to write off a whole discipline because of a few groups. GMO crops like golden rice can improve access to nutrition in developing countries and don’t pose much harm as long as they’re well managed. Besides, genetic engineering has always been about more than just crops. My favorite example of genetic engineering to bring up is the breakthrough discovery that allowed insulin to be mass-produced in bioreactors. Insulin is a life-saving drug for millions of people and it’s thanks to a team of genetic engineers who spliced insulin genes into E. coli and S. cerevisiae that it’s so accessible. I hear people criticize bioengineering as being unnatural and unhuman, but most of our research focuses on treating diseases and improving people’s quality of life. What’s more human than that?

           It’s important for scientists as well as the public to remember that every scientific discovery can have a good side and a bad side. While a lot of non-scientists are overly pessimistic about unfamiliar advances in genetic engineering, some scientists are overly optimistic. We tend to think that science is just the pursuit of truth, but it’s not that simple.. Along with reminding others that science is a force for good, we need to remind ourselves to think ethically so we can keep it that way. It’s important to reflect at every step of the way about how advances can affect the world at large. I think we all have a lot to learn from conversations like these.

 

—Jacob Kronenberg

@jbkronenberg

My First Weeks of Summer Research By Matthew Moulton

Matthew M

My name is Matthew Moulton and I am a rising senior attending The Cooper Union for the Advancement of Science Art. After I graduate I plan to work as a chemical engineer. I applied to the NYU MRSEC REU program to gain experience and to explore a different scientific field. As part of the program, I am working as a research assistant in the Montclare Lab located in New York University Tandon School of Engineering.

This laboratory focuses on protein engineering. Essentially, the researchers create proteins with a desired outcome. For the summer, I am working with the protein Q. Q’s structure is best described as a bundle of coils. Previous research has shown that at high enough concentrations Q forms fibers that cross-link to form a hydrogel. A hydrogel is a network of polymer chains that are hydrophilic. Hydrogels are used for drug delivery and tissue engineering but most hydrogels are made from synthetic polymers. Hydrogels made from proteins like Q are more bio compatible. My job is to determine the range of conditions that this gel can form under.

q Protein

Q Protein

In order to produce Q ,researchers use bacteria as the factories to produce protein. Here are the steps:

  • The first step in this process is transformation. In this step a DNA (also known as plasmid, shown below in red) that encodes the Q protein is inserted into the bacteria host or factory. For our project we use a heat shock protocol. When the bacteria are exposed to high temperatures, their cell walls become permeable, allowing for the plasmid to get into the cell. The bacteria are transferred onto plates with nutrients that contain antibiotics. Normally, antibiotics kill bacteria. The bacteria that we use are resistant to antibiotics because the DNA plasmid contains a gene or set of genes that can breakdown antibiotics. This ensures that only cells containing the DNA grow on the plate.
  • The plates are left to incubate overnight to allow the bacteria to reproduce. The following day colonies, which are small clusters of cells, are chosen and grown in a solution containing antibiotics. This solution, called media, contains the nutrients necessary for bacteria to survive and reproduce.
  • This bacterial solution is used to initiate a larger volume of media and allowed to incubate until there are a sufficient amount of duplicated cells. Afterwards, a chemical, isopropyl β-D-1-thiogalactopyranoside, is added to the mixture to trigger the production of the Q protein. Isopropyl β-D-1-thiogalactopyranoside binds to a repressor protein (in pink below) on the DNA(shown below at the operator) and changes the repressor so it can no longer bind to the DNA. Once it comes off, another protein RNA polymerase (in purple) can take its place and begin interpreting the DNA so it can produce protein.

Gene Repressor

 

While this is just the first step of my experiments, I have learned a lot. I already knew how to perform transformation from a class I took at my university but I never learned how to produce protein. I was surprised at how many steps were involved. Protein production or expression is a process that takes several hours because once isopropyl β-D-1-thiogalactopyranoside is added to the solution, one has to wait for three hours for the cells to multiply.

colony

For me, the most difficult part of protein expression was picking colonies. To pick a colony, a pipette tip is used to gently scrape a colony from the plate and place it in a tube. The first time I tried to pick a colony, I had trouble finding one because the colonies were so small so I accidentally poked through the plate!

I am still new and I make mistakes but I look forward to learning more about protein engineering. Do you have any advice to share as I learn more about protein engineers?

Let’s chat on Twitter:@matthewantonym
Matthew Anthony Moulton

The rationale behind the dual MD/PhD degree By Andrew Wang

andrew image

(Image source)

One of the first questions I get asked by many people when I tell them that I am an MD/PhD candidate is “Why?” Usually I reply with some flippant answer about stacking degrees next to my name or avoiding a job, which gets some chuckles. However, for anyone considering whether to pursue the degree, this is just part of the story.

Many authors more eloquent than me have written about the increasing need for physician-scientists. The National Institutes of Health (NIH) has put together a helpful graphic showing the pathway of a physician-scientist, whether through a dual MD/PhD degree or a solo MD. While you do not necessarily need a PhD degree in order to conduct research as a physician, you do need an MD to see patients, and the dual degree offers a number of benefits beyond either individual degree. For me the MD/PhD degree is a marriage of the humanistic and technical parts of medicine and allows me to help patients both in the future and in the present. I’m hoping it will allow me to explore topics at the intersection of medicine and technology where historically there has been a disconnect in expertise.

The sheer complexity of the human body, and its variability between individuals, makes a medical and clinical perspective very useful when designing new therapies or diagnostics. In my field of biomedical engineering, in tackling these challenges it is often easy to reduce patients to “subjects” or “users”. We are sometimes guilty of fitting a patient to a solution rather than fitting a solution to a patient. And even when a solution is designed for a problem, oftentimes there are practical and logistical considerations that prevent the solution from being usable. This is mostly just the nature of biomedical research, and not necessarily a bad thing. As an aspiring physician-scientist my goal is to keep my research grounded, and at the same time derive inspiration for it from my interactions with patients, each of whom has their individual needs and desires.

In addition, physicians have also increasingly taken on a role as educators of technology. We are asked to provide advice on everything from vaccines, a topic which we are intimately familiar with, to robotic surgery, a topic which we are perhaps less familiar with. It doesn’t help that there is a whirlwind of information, and sometimes misinformation, available online and through various media that can cloud public perception and lead to patients ignoring or distrusting the advice of their doctors.

To be sufficiently prepared to explain recommendations to patients and advise scientists alike, physicians should be intimately familiar with reading and evaluating peer-reviewed research, including basic science and translational research. For example, recently I saw a headline on social media about scientists keeping brains alive after death, and the ensuing predictable comments about Frankenstein. Physicians are tasked with seeing past the media advertisement, understanding more precisely what exactly is being done, and explaining such to interested parties. This is an often overlooked benefit of PhD training that extends beyond a specific field.

One of the biggest concerns for many people is the length, or perceived length of the program. Because an MD/PhD degree involves two complete degrees, the length of study is usually around 8 years. As I wrap up my first year however, I have to say that the time goes by extremely quickly as you study and conduct research. In addition, most combined degree programs offer some level of tuition support, often a full tuition waiver for medical school as well as a graduate stipend for the duration of your enrollment. These factors allow you to graduate medical school without needing to repay debts, and are also enough to support a modest lifestyle. If you are passionate about biomedical research and are interested in exploring your options feel free to reach out to me or another current or former student. I’m looking forward to the journey ahead.

Andrew Wang
I meant it when I encouraged you to reach out to me! Find me on Twitter at @acuteWangle

My Unexpected Venture into Science by Julia Monkovic

My Unexpected Venture into Science
By: Julia Monkovic

julia 1

Every time I visited my grandparents’ house as a kid, I was always a little scared to go into my grandpa’s office because of a giant picture of what I thought was a bug hanging on the wall. Closely followed by my dad, I grew up thinking my grandpa was the smartest person in the world. What I didn’t know about them was their common passion for STEM – both of them are chemists. Years later, this childhood vision still holds true. As it turns out, that big picture of a bug is actually the structure of an organic molecule my grandpa synthesized that’s now being used as a nausea treatment for chemotherapy patients. Starting with him and passing through my dad, science has crawled through my family and somehow made its way to me – something I never would’ve guessed just a couple of years ago.

julia 2

Despite the amount of science present in my family, as I grew older I became more and more resistant toward the field. In high school, my interests circled around reading and playing the flute – I didn’t enjoy the science classes I took, finding them boring, intimidating, and not for me. So in my senior year of high school when it came time to deciding what to study in college, my friends were so shocked I chose to go into engineering they thought I was pranking them. I laughed along with them, while at the same time being driven by fear, the unknown, and a spark of passion to create change.

That spark started not by any positive inspiration or revelation, but through a moment of frustration and despair as I watched a seemingly incurable disease take over my brother’s life. One evening while my family had another argument over which medicine or doctor to try next, I wanted to take matters into my own hands and went to Google to search for other forms of treatment available to my brother. Despite the great successes and advances in the medical field of recent decades, through this experience I felt incredibly frustrated with it at the same time. Many confusing articles later, I stumbled across biomedical engineering. At that time my feelings of anger and defeat turned into ones of pressing eagerness, as I began to believe that pursuing this field was how I could make a change in the lives of my brother and those like him.

Through this experience science began to mean more to me than just a sector of subjects in school. Rather, it’s a way of thinking that we have the potential to help heal and improve the lives of people around the world. It can give us optimism and hope in times of despair and allow us to come together to work collaboratively towards our goals, as science is a team sport rather than an individual one. Science isn’t about memorizing formulas or reaction mechanisms: it’s about asking your own questions, developing your own theories, and eventually finding your own answers to the unknown. Growing up I always felt like science “wasn’t for me,” but over time I’ve realized that science encompasses everything we do, and therefore it’s naturally a part of me.

This transition into STEM was anything but easy. Because of my lack of a science background I was worried people wouldn’t take me seriously. I was hesitant to ask questions in class for fear of sounding stupid and still struggle a lot with confidence. I gain inspiration and confidence to persist through the encouragement of my family and friends, along with the great opportunity to work in a lab that designs ways to help fight diseases like my brother’s.

As I said earlier, science is not something that can be done alone: I’m thankful for my dad and granddad for giving me a platform to grow from, and for my mentors and professors for taking a chance on me and seeing the potential in me before I saw it in myself. I now know that in order to go into the sciences, you don’t have to be the “smartest person in the world.” All it takes is a strong support group and that one spark of ambition to create change.

-Julia Monkovic
@JMonkovic