JOINIT JOY!” Osteoarthritis drug delivery research
Our Osteoarthritis drug delivery research is featured on NSF’s “The Discovery Files”
First Day
Maria Kulapurathazhe
The first day of school is something that I have always looked forward to for as long as I can
remember. Catching up with friends during lunch, writing on the first page of a new notebook,
trying not to look lost on the way to class – these are just some of the small things that make me
have a crazy smile on my face for the entirety of the day.
For obvious reasons, this past first day of school was like no other. After saying goodbye to my
roommates and the city after a fleeting six months as a college freshman, I returned to NYU
Tandon as a junior with eyes set on graduation.
Even stepping foot on campus again and exchanging small talk with classmates seemed surreal
after months of virtual backgrounds and disconnected Zoom chat messaging. With everything
around me seeming to have gone through a radical change in the last year and a half, I couldn’t
help but wonder how my experiences over the pandemic would manifest in the fall.
One of the few constants I had though this year was my time in the Montclare Lab. For the past
three months, I was able to fully immerse myself in the day-to-day activities of a bioengineer and
scientist. From figuring out how to analyze different temperature scans to running gel
electrophoresis and even growing used to the distinct smell of bacteria cells, the last few months
were a whirlwind of finding my footing in a field I am beginning to realize my growing passion
for.
Just as thrilling was being able to see everything I had learned in the lab come together in [...]
NYU Press Release:
“NYU researchers, including Jin Kim Montclare, professor of chemical and biomolecular engineering, along with investigators from the NYU Grossman School of Medicine have found both the molecular vehicle and therapeutic payload for delivering pharmacologic treatment to affected joints, halting post-traumatic osteoarthritis (PTOA) onset and progression.”
Protein Engineering Research Internship
Sihan (Hannah) Jia
I recently graduated from New York University – Tandon School of Engineering with a Master degree of biotechnology. During my time studying biotechnology, I learned to find my topics of interest and designed my own experiments. Every time I presented my research work, I gained many positive comments from my classmates and professors, I felt so motivated and therefore worked harder. I collaborated well with my colleagues and they inspired me a lot when discussing about projects. Since both of my parents work in hospitals, they taught me about medical science since I was a child. Gradually, I began to be intrigued by this amazing field that seemed like another world. So I found that I was more interested in figuring out how life works and that’s why I chose molecular biology as my career. Protein engineering is an enchanting field where altering biological characteristics becomes possible. My previous experience mainly focused on genetic engineering such as constructing and expressing recombinant plasmids in prokaryotes, protein expression and purification, western blot and ELISA. I’m really excited about practicing various new techniques.
Since completing my degree I have joined the Montclare Lab and work with Dustin and Cheng. They encouraged me a lot and guided me to be familiar with their projects. With the help of them and all other lab members, I have learned some new techniques such as syringe pump purification, CD measurement, protein concentration and BCA test. We are currently working on the optimization and application of Q proteins which can self assemble to hydrogel under normal conditions. This project is super cool and can be applied at many fields like drug delivery or conductivity.
The Solidified Path – Protein Engineering
By: Chengliang Liu
In high school I was known to be a student who really enjoyed STEM classes. As the acting president of the Math Club of my high school, I developed a deep interest in applying mathematical modeling to predict ways in which the world would evolve. I feel grateful that math has opened a new door for me to explore and enjoy the beauty of other sciences. I discovered chemistry and biology were where my true passion lay. Science has always attracted me because it is a method of problem-solving, though every subject of science has helped me to understand the world we live in better, and ultimately I choose this path for my degree.
Having this opportunity to work in the lab over this past summer has strengthened my passion for science. Experimenting with just a single plasmid that was coded for a specific targeted protein, until the pure protein is ready to be tested, is really an incredible experience. During class lectures, professors tried to teach the technical theories of science, but because of time limitations, the lecture is usually broken into fragments. In the grand scheme of things, students are not able to fully understand the reasoning behind the techniques. In lectures we always anticipate successful experiment results, however, this is not the case at all. In a research lab, more often than not this will take multiple trials until desired data can be collected. We discuss the problem we face and try to improve on the protocol, and bit by bit we figure out the issue and eventually solve it. This is what I viewed as the most rewarding part about science, and this is what motivated me to come [...]
My Journey to the Lab
By Aparajita Bhattacharya
Growing up, I was utterly fascinated with the inner workings of the living world. This fascination as well as curiosity led me to major in molecular biology during college. However, my senior year, I found that I lacked work experience in the field and didn’t know how my knowledge from class would apply in the real world. Intending to fill this gap, I landed a credit-based volunteering opportunity in a genetics lab that used Drosophila as a model organism to understand aging and Alzheimer’s disease. This lab experience not only gave me a taste of the world of science, but also helped me to get my first job as a research assistant studying the molecular mechanism of Alzheimer’s disease. We sought to map the molecular causes of protein dysregulation, which lead to amyloid accumulation in the brain, and eventually result in dementia. Since I am interested in more readily applied research, this project caught my attention. In my own small way, I was addressing a challenge facing human health by advancing the understanding of the origin of Alzheimer’s disease. Feeling fired up from my stint in the research lab, I entered a Master of Research in Molecular and Cellular Biology (MRes), which was like a mini-Ph.D. The program allowed me to assess whether I’d be interested in the commitment of a Ph.D. program.
Aparajita Bhattacharya, PhD Candidate
As a part of the MRes, I worked in two labs executing two Master’s thesis projects. In the first, I investigated the unorthodox idea of translation in the nucleus using a yeast model in the School of Biosciences. In the second, [...]
A (Not so Typical) Year of an Undergraduate Student
By: Bonnie Lin
March 11 th , 2020: NYU announced that all classes and non-essential research will be conducted
remotely as a precautionary measure for the COVID-19 issue that is getting worse. The sudden
transition into remote instruction was and still is, met with several challenges. However, classes
still managed to move forward with a combination of Zoom and online exams.
Research on the other hand is even more difficult, or even impossible, to fully transition to
remote operation. Ongoing efforts of research through remote settings increased to avoid
delay in research. Despite increased focus on literature research and computational
simulations, findings ultimately required experimental confirmation traditionally conducted
through wet bench research in a laboratory setting.
June 1 st , 2020: Remote research in computational design starts. As a Thompson Bartlett Fellow,
I worked on computationally designing phosphotriesterase (PTE) variants through incorporation
of non-canonical amino acid, p-fluorophenylalanine, to detoxify organophosphates. For ten
weeks, I was able to familiarize with the computational modeling software, Rosetta, and was
able to identify PTE candidates with increased catalytic efficiency and binding affinity with the
help of my mentor Farbod Mahmoudinobar and Dr. Montclare.
The idea of approaching protein engineering from a different perspective was full of challenge
and excitement. Both computational design through remote operation and wet bench research
are similar in two ways: that lasting feeling of accomplishment to participate and contribute to
the world of scientific discoveries and findings.
Fall 2020: Classes are being held through a wide range of flexibility to accommodate students in
the midst of a pandemic. Undergraduates are offered a choice of taking in-person classes, fully
remote or blended (hybrid version). Laboratory courses are offered in-person only and [...]
Unexpected Computational Research
By: Jason Chen
When I joined the Montclare lab for protein engineering and molecular design in the summer of 2020 (amidst a pandemic), I was eagerly expecting to be thrown into the wet lab world involving the prolific use of SDS-PAGE and the omnipresence of micropipette tips. Instead, I would find myself navigating a Unix shell often in the comfort of my bed (which was not great on my back, in retrospect). Scientific research is not all about wet lab work, as one might presume. In fact, computational work is one of the core driving forces of breakthroughs and advancements, especially in the field of protein engineering.
During my first few weeks, the learning curve was very steep. Although I was armed with three semesters of coding experience from my computer science courses, I was still mystified by the Unix interface and the modeling software called Rosetta. I perused through countless random Github and Stack Overflow web pages trying to figure out how to execute basic commands. I read and reread past papers about our protein construct (phosphotriesterase or PTE) to understand the meaning behind the work.
Most importantly, I received guidance and encouragement from my mentor, the patient and wise post-doctoral associate Farbod Mahmoudinobar. It also didn’t hurt to have worked with other undergrads on the project, Bonnie and Jakub, who sympathized with the struggles of undertaking computational work for the first time. We often connected through Zoom to troubleshoot difficulties and work together throughout the learning process.
Eventually, I grew more comfortable with the project. I gained a better understanding of the background of PTE as well as the overarching goals of our project. I was finally equipped with the [...]
From the Frontlines of COVID-19
By Joseph Thomas
I still felt incredibly groggy in the morning when I rolled over to check my email inbox on my phone. I usually joke that my brain isn’t fully “on” until I have my morning coffee and this cold April Saturday was no exception. I was staying in a Philadelphia suburb with my partner, their roommate, and their roommate’s parents. My partner was immunocompromised, and when the virus began to rapidly spread in New York City we made the decision to leave for a safer, more spread out environment. When we left there was fear that New York City would be quarantined, that the rich had been tipped off early to flee in helicopters, and that being trapped inside could be a death sentence for anyone vulnerable. Luckily those all turned out to be rumors, but the situation in the city was still dire. Only a few days prior I had sipped my therapeutic morning coffee and watched a livestream of the USNS Comfort docking in Manhattan, in anticipation of the human suffering that was soon to overflow from emergency rooms citywide.
My eyes finally focused a bit more and I saw the email I had been waiting for. “How quickly can you get back to Brooklyn? I want you to start training on how to run Coronavirus tests on Monday.” A former colleague of mine was organizing graduate students and medical students to help the overwhelmed medical staff of University Hospital Brooklyn. In the previous weeks I had emailed everyone I could think of, from hospitals all the way to the National Guard, trying to find some way I could help. As a scientist I felt so helpless just sitting [...]
Rolling with the Punches
It was December of 2019. The ground was full of snow, my student home looked like a
holiday store exploded in it, and peculiar news was circulating after a mystery disease infected a
Wuhan, China local who ate a bat; we thought it was probably fake news…
The year was coming to a close, and so was the second last semester of my undergraduate
career. Of course, my mind was tossing and turning between excitement and denial. My four
years at Queen’s University in Kingston, Ontario, shaped me into the person I have become. The
thought of starting a new journey at a new school had already crossed my mind but moving on so
soon felt as if I was cheating on the very place that I was lucky to call home. Not to mention that
my brain was fried after so many early mornings and late nights at the library, drowning myself
in coffee.
Taking a break from academics was a foreign concept that required a great deal of
consideration. I researched all the schools in Canada looking for the perfect program until one
day, my curiosity led me to the Biotechnology and Entrepreneurship master’s program webpage
at NYU. Needless to say, I fell in love with the program in an instant. I started imagining the
possibility of this new life; however, the idea did not go further. While my family was extremely
supportive of my goals, they were not quick to send me off to another country without first
applying to Canadian programs. With my lack of excitement towards what other schools had to
offer, I had decided to hold off on applying to a master’s program and take time away from
academics.
In [...]