Congratulations to Haresh More!
Congratulations to Haresh More for successful defense of his Ph.D. thesis, and best of luck in his future endeavors!
Welcome ARISE high school fellow Janill to the lab!
For more information head to http://arise.poly.edu/
Gene delivery from supercharged coiled-coil protein and cationic lipid hybrid complex
Haresh More and Joe Frezzo publish a new paper in collaboration with Dental School colleague Seiichi Yamano
[cite]10.1016/j.biomaterials.2014.05.005[/cite]
A lipoproteoplex comprised of an engineered supercharged coiled-coil protein (CSP) bearing multiple arginines and the cationic lipid formulation FuGENE HD (FG) was developed for effective condensation and delivery of nucleic acids. The CSP was able to maintain helical structure and self-assembly properties while exhibiting binding to plasmid DNA. The ternary CSP·DNA(8:1)·FG lipoproteoplex complex demonstrated enhanced transfection of β-galactosidase DNA into MC3T3-E1 mouse preosteoblasts. The lipoproteoplexes showed significant increases in transfection efficiency when compared to conventional FG and an mTat·FG lipopolyplex with a 6- and 2.5-fold increase in transfection, respectively. The CSP·DNA(8:1)·FG lipoproteoplex assembled into spherical particles with a net positive surface charge, enabling efficient gene delivery. These results support the application of lipoproteoplexes with protein engineered CSP for non-viral gene delivery.
Montclare Lab Members Take Home 6 Awards at Annual NYU-Poly Commencement Award Ceremony
Several members of the Montclare Group win awards at NYU-Poly’s annual award ceremony, held in Pfeizer Auditorium.
Manuscript accepted to Biomacromolecules entitled “Differential Effects of Cellulose Nanocrystals on Protein Diblock Copolymers”
Jennifer Haghpanah serves as first author on this published work that documents the effects of mixing a colloidal suspension of tunicate-derived cellulose nanocrystals (t-CNCs) with aqueous colloidal suspensions of two protein diblock copolymers, EC and CE, developed here in the Montclare Lab.